I have a trichrome for GMA as well, it uses green instead of aniline blue and some other modifications. Patsy
Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of gayle callis Sent: Saturday, May 30, 2009 9:01 AM To: 'Histonet' Subject: [Histonet] RE: trichrome stain on GMA bone sections Ricki, I have a Modified Oserhoff (sp) Massons Trichrome method for bone sections in GMA but it requires a really thin sections (1 um) and no microwave heating. Bob Skinner had great success with this modified staining method. There is also a publication in J of Histotechnology on this protocol, many years back. I can dig the method out of my pre-computer day files for you if you wish. Doing trichromes on GMA embedded tissues is tricky due to the nature of dyes (aniline blue) and the dye molecular weights. Often the GMA stains blue too?? Gayle M. Callis HTL(ASCP)HT,MT Bozeman MT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet