Re: [Histonet] RE: whole mouse brains

2015-04-13 Thread Catherine Simonson
Yes, it is embedding, not encasing. That's why you use the longer processing schedule for larger tissues. Brains and spines typically require longer processing schedules than other tissues due to the higher fat levels because of the myelin. For whole mouse and rat brains, so long as they are pro

RE: [Histonet] RE: whole mouse brains

2015-04-10 Thread James Watson
We also have embed mouse brains for coronal sections routinely in deep molds, yes it can be close at times. With rat brains we actually embedded in deep molds with the Mega cassette upside down so part of the brain went above the mold and into the cassette. If we needed to go through the whole