RE: [Histonet] RESCUING UNDER DECALCIFIED BONE FROM PARAFFIN EMBEDDED BLOCKS

2013-02-28 Thread Craven Peter (NHS HIGHLAND)
Fiona We have done this in the past we took the blocks back to water be melting them out, re-cassetteing and putting through a cleaning cycle on the excelsior processor then just decal as normal and reprocess, some hardening often occurs but we can get sections of them suitable for reporting. P

Re: [Histonet] RESCUING UNDER DECALCIFIED BONE FROM PARAFFIN EMBEDDED BLOCKS

2013-02-28 Thread Rene J Buesa
Go back to water (xylene or whatever "clearant" you use → graded alcohols → water) and place in decal. The results will be slightly of less quality than if the tissue was correctly decalled in the first place, but you will be able to section it. René J.  From: Fiona J Morrow To: histonet@lists.