Thanks Peggy for the helpful information about recent commercial haemalum
substitutes.
The MSDS for tango describes only an acidified solution of the dye (eriochrome
cyanine R). This would need to be mixed with a ferric salt to make a blue
nuclear stain. Iron-ECR is probably the best substitu
I believe Celestine Blue was used much more frequently in the UK in the 70s and
80s, preferred to an iron hematoxylin, in many "trichrome" stains including
Lendrum's MSB, as Peggy mentions; the nuclear staining withstanding further
differentiation during the subsequent staining from either picri
Hello
I have used Celestin Blue-Haemalum (Mayers) considerably in the past as a
substitute for Weigert's and as you say the Celestin Blue stain does not keep
and is best made fresh for each use. Our method to simplify this was to make
separate double strength solutions of the Celestin blue an
With the hematoxylin shortage of a couple of years ago (real, not imagined
in about 2007-2008), several companies tried to come up with a synthetic dye
substitute.
A little background: Celestine blue (CI 51050, also known as Mordant blue
14) is a substitute touted many years ago (late 1960s/ea
