RE: [Histonet] Re: EDTA

2010-02-22 Thread Andrea T. Hooper
Subject: RE: [Histonet] Re: EDTA To: 'Rittman, Barry R' barry.r.ritt...@uth.tmc.edu, 'Histonet' histonet@lists.utsouthwestern.edu Date: Friday, February 19, 2010, 11:32 PM We use EDTA tetra-sodium salt, pH 7.6 but the pH is adjusted down with glacial acetic acid using continuous stirring and a pH

RE: [Histonet] Re: EDTA

2010-02-19 Thread Rittman, Barry R
I agree with Gayle and Rene I would not even use a mixture of the two. Formic acid demineralization will work fine, the theory of using EDTA with it makes no sense. EDTA demineralization usually uses the disodium salt. EDTA by itself is not very soluble but its sodium salts are. It can be

RE: [Histonet] Re: EDTA

2010-02-19 Thread gayle callis
acid would have been faster and cheaper. Gayle Callis -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rittman, Barry R Sent: Friday, February 19, 2010 3:20 PM To: 'Histonet' Subject: RE: [Histonet] Re