Actually, Bob, the method the investigator sent me was the Romanovsky Giemsa that uses HEPES buffer. But it requires preparing all the reagents from scratch and the HEPES alone is several steps. I would like to buy prepared reagents. Can you buy a Giemsa kit for frozens?
Peggy -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Monday, April 04, 2011 2:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Giemsa Stain for frozens Peggy Sherwood with the Wellman Center for Photomedicine at the Massachusetts General Hospital asks: >>I'm curious what Giemsa stain method people are using for frozen sections? I have a simple Wright-Giemsa stain that I use for blood smears (using a 1 min. methanol fix, 30 second stain with Wright-Giemsa, 5 minute in Phosphate buffer and then rinse in dH20). The investigator sent me a method using a HEPES buffer. It is involved to make up the working buffer of HEPES.<< If it LOOKS good, it IS good, as Duke Ellington might have said. In surgical pathology most people use one of the two-part Romanovsky type stains - Diff-Quik or any of a number of satisfactory generic equivalents. This is a very simple stain to do. I would think it would be publishable, since the stain is well known and easily obtained. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
