I agree with the Samuri on histogel, it can be a great tool for making cell
blocks but sometimes once I get the block to the microtome either for
frozens or ffpe blocks it does not section well and can be a nightmare.
Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
You can also use agar. It does the same thing and is cheaper =)
Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas 78744
(512)901-0900 ext. 6912
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Currently doing Cryotomy on quickly frozen Golgi-Cox impregnated mouse
brains
at 100-120 microns. Cryoprotecting with 30% sucrose. Cutting at -15C.
Tried fresh tissue and brief NBF perfusion(5 min).
If I perfuse with NBF for 15 min, or immersion fix overnight, it seems to
destroy
spines and
