So this has confused me more. So before you would run a negative control
for each block you were testing and you would use the negative mouse or
rabbit reagent. Now you don't have to do that but you still need a
negative tissue control. So what exactly does this mean? Does it mean
for every
As of now, my lab took that as you just needed one negative slide per
run. And that negative slide is getting the negative mouse or rabbit
serum. Is this correct?
Tasha Campbell, B.S.,HTL(ASCP)
Frederick Gastroenterology Associates
310 W. 9th St.
Frederick, MD 21701
301-695-6800 ext. 144
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Campbell, Tasha
M.
Sent: Wednesday, April 30, 2014 6:48 AM
To: Terri Braud; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Negative Controls
So this has confused me more. So before you would
...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A
Sent: Wednesday, April 30, 2014 8:35 AM
To: 'Campbell, Tasha M.'; Terri Braud; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Negative Controls
Tasha,
We use the negative elements within our patient sample as our negative tissue
: [Histonet] Re: Negative Controls
Tasha,
We use the negative elements within our patient sample as our negative tissue
control.
Linda A. Sebree
University of Wisconsin Hospital Clinics IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
-Original Message
08:04:43 -0600
Subject: RE: [Histonet] RE: Negative Controls for IHC
I just stopped using the reagent controls on all our IHC due to the drastic
cuts in reimbursement. It will be a significant immediate savings for our
tiny lab.
Cheryl A. Miller HT ASCP cm
Histology Supervisor
Hygiene
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver
Sent: Wednesday, February 12, 2014 11:04 AM
To: Cheri Miller
Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Negative Controls for IHC
Cheryl
Yes, I
Not too long ago, there was a posting about labs that had discontinued. You
technically don't need with a polymer, non-biotin system. I still do them- for
now. The pathologist never uses them, internal controls only. But I do, but
this may be a good cost cutting idea for the future.
Joelle
I was also taught to run a positive and negative every time you do an
immuno stain, provided there is sufficient tissue. I was trained at AFIP, by
the people that wrote the second AFIP staining manual (the red one). Of
course, if cost is your number one concern.
