Try Goldner's Trichrome Stain. I have protocol if you want -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sean McBride Sent: Thursday, May 07, 2009 8:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Request for Staining Advice: Rabbit Calvaria & underlying Cerebral Hemishpere
Colleagues, I have been charged with the task of evaluating an hydroxyapatite coated biomaterial implanted in the calvaria of a rabbit model. The calvaria and cerebral hemisphere were excised en bloc, and the objective of the experiment is to assess not only new bone formation and osseointegration but also any pathological effects on the dura mater, arachnoid, pia mater and underlying cerebral hemisphere. I have assessed both brain and bone individually in the past, but never en bloc. The specimens have been embedded in pmma and mounted for thick ground section (~30 - 35 microns) evaluation. Does anyone have any suggestions for staining techniques that will show both bone & brain morphology in an en bloc specimen? Kind regards, ~Sean McBride Senior Researcher Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 412-268-8275 (o) 412-901-7540 (m) 412-268-8641 (fax) smcbr...@cs.cmu.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet