How old the sections are may give you some clue about if they were initially
mounted on (+) slides or not.
In any event, I would try to soften the mounting medium first with heat. Try to
displace the coverslip with your finger when the mounting medium softens. It
usually does.
If not, use warm x
Have you tried baking or placing your slide on a slide warmer for a few hrs or
overnight prior to staining? That seems to help make the sections stick.I would
do this step after transferring the section to a charged slide.
-Mehlika
> From: kst...@mcw.edu
> To: histonet@lists.utsouthwestern.edu
