We have used the antibody from abcam on FFPE mouse tissue with good
success, have not tired it on frozen sections
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949
fax (303) 682-9060
www.premierlab.com
Ship
...@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala
Sent: Monday, November 23, 2009 4:28 PM
To: Richard Cartun; Histonet
Subject: RE: [Histonet] TNF alpha
Richard it works. We purchase the antibody from Abcam.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box
Sorry Guys I was thinking of IL-6 which I have done on bone and it
works. We have worked with TNF-alpha (from Sigma) and our protocol does
not requite retrieval I would think it would work on formic acid
decalcified bone also, but I have not tried it.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Richard it works. We purchase the antibody from Abcam.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949
fax (303) 682-9060
www.premierlab.com
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1567 Skyway Drive, Unit E
Longmont, Colorad
