Hi Bernice,
Likely there is something in your TUNEL procedure that is causing a problem
with histochemical nuclear staining. If it isn't the pre-treatment, it might be
the DAPI. After all, they do bind the same structures, and the DAPI might be
winning that competition.
Teri Johnson
Manager,
I have done that a lot and I don't see why it would not pick up hx or DAPI.
Was there anything left on the slide? Maybe it was mounted in aqueous
already??
mills
On Fri, Mar 3, 2017 at 11:42 AM, Bernice Frederick via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> Hello all.
> Would
We use the Roche Kit with good success.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949
fax (303) 682-9060
www.premierlab.com
Ship to Address:
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Longmont, Colorado 80504
Gil,
I routinely incubate free-floating 40um rat brain sections overnight or
longer @ 4 degrees centigrade according to the particular antibody that
I am working with. Our sections are perfused in 4% paraformaldehyde and
cryoprotected with 30% sucrose prior to sectioning. How long are you
Human brain? I prefer Cleaved Caspase 3 to Tunnel, in my experience Tunnel
does not distinguish apoptosis from necrosis but CC3 will only label cells
under going apoptosis.
Patsy
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Guillermo
We have not used that kit specifically we use the one from Roche but we get
fine results with the following fixatives, 10% NBF, 4% Paraformaldehyde and
Davidisons, its also works fixed and EDTA decalcified samples. You will need
to add a proteinase K step to your protocol. You need
