From: "Swain, Frances L"
Date: Wed, 18 Mar 2009 08:12:45
To: Dr. med. Frauke Neff;
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] cryoprotection possible?
Usually the cryoprotection is carried out after the specimens are
fixed and before they are frozen. If you
rauke Neff;
histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] cryoprotection
possible?
Good advice indeed. However, I don't recommend you rinse in water after
sucrose. Sort of defeats the purpose. Instead if you need to remove
excess sucrose rinse in either 50/50 sucrose/OCT or
Sent: Wednesday, March 18, 2009 8:50 AM
To: anh2...@med.cornell.edu; Dr. med. Frauke Neff;
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] cryoprotection possible?
You are so correct, I forgot you do not rinse the sucrose out you blot it and
then freeze. Sorry about that. I do not cut
. med. Frauke Neff; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] cryoprotection possible?
Good advice indeed. However, I don't recommend you rinse in water after
sucrose. Sort of defeats the purpose. Instead if you need to remove excess
sucrose rinse in either 50/50 sucrose/O
ve to consider whether your fixation is appropriate for your
antigen/antibody.
-Original Message-
From: "Swain, Frances L"
Date: Wed, 18 Mar 2009 08:12:45
To: Dr. med. Frauke Neff;
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] cryoprotection possible?
Usually the
Usually the cryoprotection is carried out after the specimens are fixed and
before they are frozen. If you have a sample you can spare you might try
making up some 20% Sucrose placing the frozen sample in it. putting it in the
refrigerator and letting it stay in the 20% sucrose until it drops t
