Andi, you may want to contact Bob Skinner directly with your questions. skinnerrobe...@uams.edu
I worked with Bob for about twelve years and he was and as far as I know is still using methyl salicylate in place of xylene as a clearing agent. Methyl salicylate avoids the brittleness and hardening that can occur with xylene. I don't know if he's compared his clearing technique with Clear Rite. Bob's work is in orthopaedics, I don't know if he's done much with teeth recently but I know he did some eons ago. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Andrea Grantham Sent: Tuesday, February 24, 2009 2:55 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cutting teeth I'm hoping that this isn't as painful as the actual process we all went through as children. I'm told that one of the investigators here will be sending a couple hundred teeth to my lab soon. I have never processed or sectioned teeth before so I'm hoping that I can find a few good suggestions on histonet. Here is what they are doing - based on two papers from JOE in 05 and 07: Fixation is in 10% NBF - how long do you fix teeth? Is temperature something to consider here? Decalcification is done in a solution of equal parts of 50% formic acid and 20% sodium citrate. The papers say 10 days is enough to decalcify teeth. Is it enough time? Following decal they infiltrated the teeth using "Skinner's method". Anybody know what this involves? One paper says Methyl salicylate was used as a clearing agent. Why is this better than traditional processing on my VIP using Clear Rite 3 as a clearing agent? In the papers the teeth were embedded in Paraplast and sectioned at 5 microns and then thinner, they actually want thinner sections - like 2 microns. We are looking for pulp tissue. Then they want a Brown and Brenn stain and maybe a trichrome. Thanks, Andi ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algra...@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet