Hi Meryl,
What stain are you using your Hematoxylin for? We use Harris Hematoxylin for
HE staining and we filter it each day that we need to use it.We change it
once per month. We are also not a high volume lab. We are a histology
department in a biotech company. Your suspicions are
We are a small lab and used Vintage for a while and loved the stain. I did
notice that both the Eosin and Hemat did become watered down faster than any
other brand we had tried and evaporated more. I also could only use it for
about 2 wks before having to toss and refill. If it was more cost
Meryl,
We uses for more than 15 years a home made regressive
hematoxylin Lillie. Our lab stains approximately
5000-6500 slides monthly. We does our stains maually.
We never change our solution, but time to time add new
hematoxylin for necessary level in stainig dish.
Everyday we filter our working
Absolutely it becomes diluted from water - are you hand staining or auto?
Autostainers tend to allow a longer time for excess water to drip off - more
consistency. Are you covering your htox when not in use? Do you notice any
type of sheen on the surface prior to staining? Have you noticed
