Giemsa is what we use routinely for mast cell neoplasms and normal mast cells.
For us it works equally well as T blue for diagnostic purposes. Our positive
control is a cutaneous mast cell tumor (very common in out animal species), or
skin biopsy with allergic dermatitis.
Poorly
If you don't want the with purple-red metachromasia, try neutral red, which
gives an orange-red metachromasia and true red nuclei.
John Kiernan
Anatomy, UWO
London, Canada
= = =
On 01/11/11, Patsy Ruegg pru...@ihctech.net wrote:
Does anyone have a go to stain for Mast Cells besides Tol
Breast fibroadenoma is a great mast cell control
Rosie Scrimizzi
Oz
Sent from my iPad
On 03/11/2011, at 1:27 AM, monet...@mskcc.org wrote:
Giemsa is what we use routinely for mast cell neoplasms and normal mast
cells. For us it works equally well as T blue for diagnostic purposes. Our
Go to http://stainsfile.info/StainsFile/stain/cell/aldtolblue.htm for a
reliable method. It still uses toluidine blue, but the staining is not
based on metachromasia and mast cell granules are dark blue. Most
toluidine blue samples will work, but occasionally one doesn't. Fisher
brand is OK as
A good one that I used to use was the Naphthol AS-D Chloroacetate-esterase
which stains the non specific esterase activity in mast cells. It is a
pain to run, but it is one of the most beautiful. A bright red mast cell
against a pale background. We got it from Vacca, p 530-533.
On Tue, Nov 1,
