If you drop the condenser down so Koehler illumination is not aligned, this give you a pseudo phase contrast. You can see many structures in tissue due to the different density of the cells. So I would recommend a microscope that has a condenser that can be moved up and down. We have 2 old Olympus BH2 in our Microtomy area.
James Watson HT ASCP GNF Genomics Institute of the Novartis Research Foundation Tel 858-332-4647 Fax 858-812-1915 jwat...@gnf.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, February 27, 2014 9:57 AM To: Histonet@lists.utsouthwestern.edu; Lisa*Freeman Subject: Re: [Histonet] microscope information ANY microscope with decent objectives will do. Even used microscopes you can buy in eBay. The problem is not with the microscope but how the HTs will be able to locate those structures while still embedded in the paraffin. René J. -------------------------------------------- On Thu, 2/27/14, Freeman, Lisa* <lisa.free...@fda.hhs.gov> wrote: Subject: [Histonet] microscope information To: "Histonet@lists.utsouthwestern.edu" <Histonet@lists.utsouthwestern.edu> Date: Thursday, February 27, 2014, 12:46 PM I am currently looking for a microscope to use at the sectioning stations. We have to locate tissues such as parathyroid glands and mammary glands at sectioning, as well as general QC. Any opinions on which is the best for this purpose? Thank you, Lisa Freeman, HT Histology Supervisor Toxicologic Pathology Associates National Center for Toxicological Research 3900 NCTR RD Jefferson, AR 72079 Phone: 870-543-7234 E-mail: lisa.free...@fda.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
