You are probably going to have to check unstained sections as you trim into the block. The heart needs to be grossed in from apex to base in a standard manner, you will need to figure out the best way to do this. You need to section on the same orientation each time. I'm thinking you need to section in the frontal plane, trim off a small portion of the anterior side of the heart, you can tell the anterior from the posterior by the atria. The atria are attached to the heart on the posterior side, so you want to trim off the other side, we have done this for rat hearts before to look specifically at the papillary muscle. I would then section into the block and then pick up unstained slides and look under a microscope to see if the papillary muscle is present. Good Luck
Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of abijag Sent: Saturday, August 13, 2011 2:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] mouse heart trimming and sectioning_papillary muscle Dear fellow histonetters, I would like to have your suggestions regarding trimming and sectioning of mouse heart. Our pathologists are interested in evaluating mouse ventricle papillary muscle for some lesions. I am not able to cut sections having papillary muscle in all sections.Some sections I am getting but not in all. Anybody experienced this situation and any experience to share. With regards Abi _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
