Does anyone know of a method to change the pH of tap water without going through a lot of expense? Possibly a filter to put directly on the water line. Thanks, Toni
-----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Matthew Close Sent: Monday, March 23, 2009 12:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] re: water I think that distilled water starts out at a ph of around 6-6.5, but usually DI water is closer to 7. Over time, both will drop in pH unless you buy specialized housing for the DI water. Since many people doing IHC at my university are using RO water, which has a pH similar to what the pH of the tap source, I would say you are probably okay going with the distilled water. My word of caution is to check the level of air in your housing tank and never let it drop below half. Typically, the more air in the tank, the more CO2 going into the water which will gradually lower the pH down to 5.1-5.5 territory. Also, if you have the option to chose between using a glass carboy and a plastic one to store water, always go with the glass. While all of this has never been a real problem for me in terms of analyzable results, I often wonder if it has anything to do with why, sometimes, identical staining procedures on identially fixed tissues will stain notably different. Matt Close Department of Biological Sciences Lehigh University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more.
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