Re: [Histonet] Tissue processing

2020-03-06 Thread Patpxs via Histonet
I favor the VIP too. My lab has 4 VIPs and 1 Leica Peloris. The VIPs can process more cassettes on a run and cost a lot less than the Peloris. Paula Sent from my iPhone > On Mar 6, 2020, at 12:34 AM, warda hassan via Histonet > wrote: > > Hello to all histonets > > Advanced thanking t

Re: [Histonet] Tissue processing question

2016-01-29 Thread Michael Ann Jones via Histonet
e to use. > >From: Caroline Miller [mailto:mi...@3scan.com] >Sent: Friday, January 29, 2016 1:36 PM >To: Walter Benton >Cc: Charles Riley ; histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] Tissue processing question > >I really like this type: >https://www.fishe

Re: [Histonet] Tissue processing question

2016-01-29 Thread Shirley A. Powell via Histonet
ry 29, 2016 1:36 PM To: Walter Benton Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Tissue processing question I really like this type: https://www.fishersci.com/shop/products/starplex-scientific-histoplex-tissue-cassettes-micromesh-chamber-8/p-2782584 (although I buy them from maste

Re: [Histonet] Tissue processing question

2016-01-29 Thread Caroline Miller via Histonet
I really like this type: https://www.fishersci.com/shop/products/starplex-scientific-histoplex-tissue-cassettes-micromesh-chamber-8/p-2782584 (although I buy them from mastertech, but they seem to have dissapeared from their website) They are great for both large tissues, and also biopsies. A lon

Re: [Histonet] Tissue processing question

2016-01-29 Thread Walter Benton via Histonet
. From: Caroline Miller [mailto:mi...@3scan.com] Sent: Friday, January 29, 2016 1:36 PM To: Walter Benton Cc: Charles Riley ; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Tissue processing question I really like this type: https://www.fishersci.com/shop/products/starplex-scientific

Re: [Histonet] Tissue processing question

2016-01-29 Thread Walter Benton via Histonet
We use hair wrapping paper used for perms. It is the same paper called "biopsy wraps," but at a significant price reduction. You can buy a variety of sizes and the wraps do not cause artifacts and are porous enough for ample solution penetration. Biopsy paper comes in blue and other colors, but

Re: [Histonet] Tissue processing question

2016-01-29 Thread Rene J Buesa via Histonet
Sponges can cause a compression artifact leaving some sort of "imprint" on the surface of the biopsy, especially kidney and prostate Bx.I my experience tissue paper is the best option. If you are having difficulties with the wrapping, you can use "tea bags".René  On Friday, January 29, 201

Re: [Histonet] Tissue processing question

2016-01-29 Thread Walter Benton via Histonet
We use hair wrapping paper used for perms. It is the same paper called "biopsy wraps," but at a significant price reduction. You can buy a variety of sizes and the wraps do not cause artifacts and are porous enough for ample solution penetration. Biopsy paper comes in blue and other colors, but

Re: [Histonet] Tissue processing validation

2015-07-21 Thread Tony Henwood (SCHN) via Histonet
The validation would already have been done. You only need to check if the re-location has affected any equipment components. I would take a few cassettes of spare tissue and process it on your most used program and await the results. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT

Re: [Histonet] Tissue processing for laser microdissection and RNA isolation?

2013-02-13 Thread Rene J Buesa
If you fix adequately (no NBF) you can go with 1 René J. From: Mikael Niku To: histonet@lists.utsouthwestern.edu Sent: Wednesday, February 13, 2013 8:15 AM Subject: [Histonet] Tissue processing for laser microdissection and RNA isolation? Hello! May I ask for your recommendations for tissue p

Re: [Histonet] tissue processing problem

2011-07-14 Thread Rene J Buesa
You are right, it is most likely a dehydration issue. Try to start with 70EthOL and increase gradually until 100EthOL René J. --- On Thu, 7/14/11, Carol Bryant wrote: From: Carol Bryant Subject: [Histonet] tissue processing problem To: "histonet@lists.utsouthwestern.edu" Date: Thursday, July

RE: [Histonet] tissue processing

2010-03-17 Thread Liz Chlipala
Sheri Your processing cycle is too long. We process 20 minutes per station for mouse tissue only 2 absolutes and 2 xylenes and 3 paraffins. Even 3 absolutes and 3 xylenes at 20 minutes a station we find over processes the tissue. Liz From: histonet-boun...@