If I am not mistaken vectamount is an aqueous mountant, dpx is xylene/toluene based and requires going thru alcohols and xylene to mount. One thing I do and you can try it, is airdry the slides and the use permanent mounting media without going thru alcohols and xylene, keep in mind there is a little solvent in the media so it may have an effect. I do this with Fast Red substrate chromogen for AP have not tried it on vector blue. The other option is to use an aqueous mountant and seal the coverslip edges with clear nail polish. Patsy
Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of anjan kumar Sent: Wednesday, March 04, 2009 12:05 PM To: histonet-reque...@lists.utsouthwestern.edu; triple immunohistochem Subject: [Histonet] vector blue- xylene soluble- any alternatives hello everyone, i have a problem which i dont know how to tackle, the problem is that i have purchased vector blue as a chromogen and it is xylene soluble can anyone suggest me how to avoid this.... as funds are low i cant purchase xylene substitutes. second question been, ...sorry this has been a addition ...... what is the difference between the DPX mountant and other permanent mounting media like vectamount. kindly answer these questions... regards, Anjan Kumar Junior research scientist Veterinary Pathology Madras veterinary college. _________________________________________________________________ Ready for MARRIAGE? Join MSN Matrimony! http://www.shaadi.com/msn/matrimony.php?ptnr=msnhottag______________________ _________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet