Re: AW: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde

2013-12-09 Thread Orla M Gallagher
...@lists.utsouthwestern.edu] On Behalf Of pru...@ihctech.net Sent: Thursday, December 05, 2013 2:50 PM To: gu.l...@gmx.at; 'Orla M Gallagher' Cc: histonet@lists.utsouthwestern.edu Subject: RE: AW: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde i would think u are correct

Re: AW: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde

2013-12-09 Thread Barry Rittman
...@ihctech.net Sent: Thursday, December 05, 2013 2:50 PM To: gu.l...@gmx.at; 'Orla M Gallagher' Cc: histonet@lists.utsouthwestern.edu Subject: RE: AW: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde i would think u are correct in advising formic acid decal

RE: AW: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde

2013-12-09 Thread Sally Ann Drew
@lists.utsouthwestern.edu; Wineman, Terra Subject: Re: AW: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde hi I would recommend storage for long term in 70% ethanol. To prevent drying out we used glycerin in the ethanol, about 20% of the volume. Barry On Mon, Dec 9

RE: AW: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde

2013-12-06 Thread Wineman, Terra
To: gu.l...@gmx.at; 'Orla M Gallagher' Cc: histonet@lists.utsouthwestern.edu Subject: RE: AW: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde i would think u are correct in advising formic acid decal and then processing into paraffin for the best protection

RE: AW: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde

2013-12-05 Thread pruegg
i would think u are correct in advising formic acid decal and then processing into paraffin for the best protection of the trap enzyme, immunoreactivity, etc. A couple of weeks in formalin should be fine. Paraformaldehyde show be the same as formalin. I do know a way to restore the enzyme