I do this all the time, but I do put a coverslip over the section and keep
it in a humid chamber. We leave it on the counter with a diaper over it and
an ice pack sitting on top.
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
w
I agree with Kim, your cytoplasmic staining with hrp/dab is probably because
your anti mouse detection is reacting to endogenous Mouse IgG in the mouse
tissue you are using.
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihc
