Of course you can always do IHC on your rat that died, you just have to note that that rat died and was not profuse fixed so that the review of the results will take that in consideration, hopefully you have another rat that besides dyeing and not getting profuse fixed had all other conditions the mostly the same so you could use the properly treated rat as a standard to compare the dead rat too.
Good luck and do keep asking for help at this forum, most of us will offer you our experience without judging your science. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Saturday, November 28, 2009 11:17 AM To: Salim Yalcin Inan Cc: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: RE: [Histonet] a basic question about immunohistochemistry Dear Salim, As you have been informed, doing immunohistochemistry is possible on this tissue. After all it's possible to do IHC on any tissue whether the conditions you want to test under are ideal or not. Being chastised on this list and calling your work "bad science" is totally out of line and certainly does not help you out. I think some people would do well to reserve judgment, particularly when there's no way they can fully understand what's going on with your project. Having worked in research myself, I completely understand that animals will die, at the most inconvenient times, during a study. First of all you should incorporate the data about the animal dying into your study notes. Secondly, there's no harm in running the IHC on this animal's tissue. You can use the results comparatively with results from some perfused tissue later on. I don't know Salim, some people might call it damage control, or making the best of a less than ideal situation. Again, I don't know exactly what you're working on but it seems there's information worth gathering despite the circumstances. I also understand that it's probably next to impossible to carry out experiments and research alone. Having reliable staff assist you is not unusual either. Good luck to you, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjas...@copc.net -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Salim Yalcin Inan Sent: Friday, November 27, 2009 2:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] a basic question about immunohistochemistry Dear All, Because I am new in immunohistochemistry, I have a basic question about it. What if your rat dies in the evening or in the weekend, which you are doing a chronic experiment and need to collect brain tissue for immunohistochemistry? And let's say, the staff did not noticed it to inform you on time. Several hours passed since your rat died. There is no way to do perfusion. Is it still possible to do immunohistochemistry? Thank you very much in advance. Best regards, Salim Yalcin Inan, Ph.D. (post-doctoral fellow) Department of Clinical Neurosciences University of Calgary syi...@ucalgary.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
