Very surprising!
I just use PFA fixation after I cut the tissue, and I can stain it again!
Anyone has the experience of staining IBA1 on alcohol/acetone-fixed brain
sections before?
In literature it said yes this works.
------------------ Original ------------------
From: "TF"<ti...@foxmail.com>;
Date: Thu, Nov 26, 2009 10:26 PM
To: "Histonet@lists.utsouthwestern.edu"<Histonet@lists.utsouthwestern.edu>;
Subject: [Histonet] IBA1-microglia staining on frozen sections
Hi all, i just can not get this IBA1 (marker of microglia) well stained on my
frozen sections fixed with acetone (sections were either dried up in air for 1
hour or fixed immediately). But I always get good staining on PFA-perfused
brain tissue.
Will IBA1 antigen reactivity lose in acetone fixation?
2009-11-26
TF
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
