I read one paper or abstract recently where sections were made at 50
microns then used
successfully for RT-PCR. I am pretty sure that it was PRRS virus that
they were pursuing.
On 3:59, Mark Tarango wrote:
I would try getting more sections from LCM and extracting into 7.5 uL, if
that
Have you tried using more sections during extraction? Can you extract into
a smaller volume?
On Friday, November 2, 2012, Vanessa Orsini wrote:
Hello,
I need to extract RNA for a RT-PCR after Laser Micro
Dissection on xGal stained slides.
I tried using sections from unfixed frozen
I would try getting more sections from LCM and extracting into 7.5 uL, if
that volume will be enough to for your PCR reactions.
On Friday, November 2, 2012, Vanessa Orsini wrote:
With the kit I'm extracting in 10ulthe problem is that I have too use
few stained cells isolated with the
Have you tried to extract the RNA from the tissue without the stain- just to
test your method? I am not familiar with the mechanics of the stain but you
might be denaturing the RNA. Also are you sure there is enough message in the
area you are trying to extract to get enough RNA? Maybe you
