Dear colleagues, I am pleased to inform you that a new article regarding a development of a novel method to study multiple steroid hormones from grey seal pup plasma was published recently.
Lea Sait, S.T., Survilienė, V., Jüssi, M., Gonzalez, S. V, Ciesielski, T.M., Jenssen, B.M., Asimakopoulos, A.G., 2022. Determination of steroid hormones in grey seal (*Halichoerus grypus*) blood plasma using convergence chromatography tandem mass spectrometry. Talanta 124109. https://doi.org/https://doi.org/10.1016/j.talanta.2022.124109 The link to the open access article can be found here: https://www.sciencedirect.com/science/article/pii/S0039914022009055?via%3Dihub Abstract A hybrid solid phase extraction (HybridSPE) protocol tailored to ultra-performance convergence chromatography tandem mass spectrometry (UPC2-MS/MS) was developed for the determination of 19 steroid hormones in grey seal (*Halichoerus grypus*) blood plasma. In this study, the protocol demonstrated acceptable absolute recoveries ranging from 33 to 90%. The chromatographic separation was carried out using a gradient elution program with a total run time of 5 min. The method repeatability ranged from 1.9% to 24% for most target analytes and the method limits of quantification (mLOQs) ranged from 0.03 to 1.67 ng/mL. A total of 9 plasma samples were analysed to demonstrate the applicability of the developed method in blood plasma from a wildlife species. A total of 13 steroid hormones were quantified in grey seal pup plasma. The most prevalent steroids: cortisol, cortisone, corticosterone, 11-deoxycortisol, progesterone and 17α-hydroxyprogesterone were detected at concentrations in the range of 9.09–40.1, 7.10–24.2, 0.742–10.7, 1.06–5.72, 0.38–4.38 and <mLOQ - 1.05 ng/mL, respectively. To our knowledge, this is the first study to determine steroid hormones in the plasma of pinnipeds using convergence chromatography. Best regards, Vaida Survilienė Junior research fellow Institute of Biosciences Vilnius university +37066211047
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