Re: [Mauve-users] Mauve's Approach To Multiple Alignments

2015-11-17 Thread Aaron Darling
Hi Rajeh, I suggest you read the algorithm description in this paper: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0011147 which discusses this issue. On Tue, 2015-11-17 at 17:09 -0600, Ahmad Rajeh wrote: > Hello, > > I have a question about Mauve’s algorithm: How does Mauve

[Mauve-users] Mauve's Approach To Multiple Alignments

2015-11-17 Thread Ahmad Rajeh
Hello, I have a question about Mauve’s algorithm: How does Mauve deal with contigs that are found in more than one region in the reference? I tried to test this by aligning a contig I had to a reference containing the same contig twice. Mauve only aligned the contig to the first occurrence in t

Re: [Mauve-users] Query regarding Mauve contig mover

2015-11-17 Thread Aaron Darling
Hi Swati, Based on that console log it looks like you may need to run some sequence assembly prior to applying the mauve contig mover. The sample names appear to correspond to short read archive accessions and the total sequence lengths are much too large to be S. aureus, which typically have gen

Re: [Mauve-users] 2 Questions: LCB and Exporting Contigs With Multiple Alignments

2015-11-17 Thread Aaron Darling
Hi Rajeh, On Mon, 2015-11-16 at 17:55 -0600, Ahmad Rajeh wrote: > Hello Mauve-users, > > I have 2 questions: First, could someone please explain seed weight > and minimum LCB to me? The manual's descriptions are unclear to me. > For example, all I want is to consider contigs that share at least 1

Re: [Mauve-users] (no subject)

2015-11-17 Thread Aaron Darling
Hi Varduhi, Based on the information you have provided it is not clear why progressiveMauve would have terminated prematurely. I note that the stdout and stderr were directed to two files, mauvetrialoriginal.e358216 and mauvetrialoriginal.o358216 I suggest inspecting those files for clues and als

[Mauve-users] Query regarding Mauve contig mover

2015-11-17 Thread Swati Agarwala
Hello, I am trying to align 26 *Staphylococcus aureus *samples in Mauve Contig Mover .the program gets terminated at 99%. How to resolve it? Running alignment. Executing win64\progressiveMauve --output=G:\MRSA _FASTA sequence- Backup\FASTA format\output\Mauve Output --output-gui