Hi Guys I am curious to know one thing that, I sterilised the LB media (which was little more diluted). I inoculated the cultures with suitable antibiotics and while I was measuring the OD of bacteria I could smell some medicine was there from the media, I really dont understand why it was like that, but not like earlier. After the OD was 0.55, i induced with IPTG but unfortunately i could nt able to express the protein.
The only mistake I did was LB media was little diluted, except this, I think I have done everything right... With curiosity, I would like to know what could be the problem? If any one has any idea...... Thanks in advance -- Sudheer Babu.S Ph.D student Institute of Biochemistry Biological Research Center Szeged,Hungary. _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
