Dear Researchmates
I isolated plasmid from zymogen plasmid isolation kit. After pellet down the bacterial cells, I added 7x lysis buffer(kit reagent) and by mistake I resuspend the pellet with pipette( suppose I should not resuspend with pipette). But when I measure plasmid concentration it was 500 ng/mico liter. I didnt check the plasmid by running agarose gel. One of my labmate told chormosomal DNA also could have come when I resuspend with pipette. When I did bacterial transformation with this plasmid I got few colonies. Could any please tell, did I do any mistake. Shall I do further steps. -- Sudheer Babu.S Ph.D student Institute of Biochemistry Biological Research Center Szeged,Hungary. _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
