http://www.nzherald.co.nz/index.cfm?c_id=2&ObjectID=10116849

Blow-in-the-bag test for terrorism
 
24.03.05
By STEVE CONNOR
 
Scientists in America are developing a way of testing the breath to
see if someone has been exposed to a biological warfare agent during a
terrorist attack. 

Dr Joany Jackman of Johns Hopkins University in Baltimore said the aim
was to detect infection rapidly after potential exposure by simply
sampling exhaled breath rather than taking blood. 

"We want to have a tool that can help in the emergency room or ... on
site so that people who are infected can get treatment first. It's not
so hard to sample breath from many people very quickly as it is to
draw blood." 

The test exploits the fact that when the body is exposed to
disease-causing organisms the immune system generates the release of
proteins called cytokines which help to identify and fight the infection. 

The researchers believe that cytokines may work their way up through
the tissues until eventually they are breathed out with water vapour
in exhaled breath. 

If so, it should in theory be possible to detect the chemicals with a
specially adapted breathalyser. 

"Old medical texts, in the days long before sophisticated diagnostics,
would recommend that a doctor check a patient by checking his or her
breath, so we knew there must be something to it," 

Dr Jackman says. 

Studies on pigs exposed to different infectious agents have
demonstrated that the technique may work in humans. 

Dr Jackman and her colleagues collected breath samples from the pigs,
and put them through an instrument called a mass spectrometer which
could test for the presence of cytokines and other proteins. 

The scientists detected a strong surge in cytokines in exhaled breath
in as little as an hour after exposure, and long before any visible
symptoms appeared. 

They also tested breath samples from disease-free pigs and found that
the markers of infection were at or below the limits of detection,
showing that it was possible to use the test to distinguish healthy
animals from those that had recently been exposed to an infection. 

The next step is to carry out tests on humans. 

- INDEPENDENT









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