I would like to attach two proteins with a disulfide linkage via two cysteine
residues I introduce using the mutation wizard. I know the 'bond' command can
be used for intramolecular disufides, but is there a way to introduce
intermolecular disulfides?
Thanks
JK
Hi Julianne,
put the two molecules into one object, preferably with different chain
identifiers. Example:
alter molecule1, chain=A
alter molecule2, chain=B
create combined, molecule1 molecule2
delete molecule1 molecule2
Now you can bond the two atoms.
You can also use the fuse command which