[PyMOL] Attachment of two proteins via disulfide

2012-05-02 Thread Julianne Kitevski
I would like to attach two proteins with a disulfide linkage via two cysteine residues I introduce using the mutation wizard. I know the 'bond' command can be used for intramolecular disufides, but is there a way to introduce intermolecular disulfides? Thanks JK

Re: [PyMOL] Attachment of two proteins via disulfide

2012-05-02 Thread Thomas Holder
Hi Julianne, put the two molecules into one object, preferably with different chain identifiers. Example: alter molecule1, chain=A alter molecule2, chain=B create combined, molecule1 molecule2 delete molecule1 molecule2 Now you can bond the two atoms. You can also use the fuse command which