Re: [R] Reading FCS files with flowCore package

2014-11-24 Thread William Dunlap
> or to change the default to mustWork=TRUE, since there are not > many use cases for querying a non-existent system file? There are a fair number of packages depending on the current semantics. E.g. ./proxy/R/dissimilarities.R:if (system.file(package="cba") == "") ./openNLP/R/pos.R:

Re: [R] Reading FCS files with flowCore package

2014-11-24 Thread Martin Morgan
On 11/24/2014 11:38 AM, William Dunlap wrote: If help files used the mustWork=TRUE argument to system.file() this sort of problem would become more apparent to the user. It would give a clear error message from or to change the default to mustWork=TRUE, since there are not many use cases fo

Re: [R] Reading FCS files with flowCore package

2014-11-24 Thread William Dunlap
If help files used the mustWork=TRUE argument to system.file() this sort of problem would become more apparent to the user. It would give a clear error message from system.file() instead of a mysterious error about file "" not being valid or, worse, a hang from an input command waiting for the use

Re: [R] Reading FCS files with flowCore package

2014-11-24 Thread Jeff Newmiller
Wrong list. See http://www.bioconductor.org/help/support/ --- Jeff NewmillerThe . . Go Live... DCN:Basics: ##.#. ##.#. Live Go... L

Re: [R] Reading FCS files with flowCore package

2014-11-24 Thread Martin Morgan
On 11/24/2014 06:18 AM, Luigi wrote: Dear all, I would like to use the R's Bioconductor package flowCore to do flow cytometry Please address questions about Bioconductor packages to the Bioconductor support site https://support.bioconductor.org and... analysis. I generated a FCS file us

[R] Reading FCS files with flowCore package

2014-11-24 Thread Luigi
Dear all, I would like to use the R's Bioconductor package flowCore to do flow cytometry analysis. I generated a FCS file using the file>export function of the FACSDiva Software Version 8 from a BD LSRII machine. I then used the functions: file.name <-system.file("extdata", "cd cells_FMO 8_0