Hello,
I have a significant problem when converting files generated with Velos (no
Orbitrap behind) from raw to mzxml to mgf. After running Petunia I end up
with mgf files of 100 MB and other of just 1 kB! All input files are
generated from identical sample, injected from identical vial and have
Oliver,
What parameters did you use to run XPRESS? The GUI showing elution
profiles has no current support for the isotope option (summed
intensities of first N isotope peaks) but otherwise should return the
same ratios as that shown in the pepXML file.
- Jimmy
On Mon, Nov 22, 2010 at 5:09 AM,
Hi,
I am not able to run ProteinProphet. I get following error:
c:\Inetpub\tpp-bin\ProteinProphet
c:/Inetpub/wwwroot/ISB/data/PeptideProphetOutput.pep.xml
c:/Inetpub/wwwroot/ISB/data/interact.prot.xml
--
ProteinProphet (C++) by Insilicos LLC and LabKey Software,
Try using wget to get the file in pieces:
http://www.cyberciti.biz/tips/wget-resume-broken-download.html
2010/11/22 钟传奇 andy...@gmail.com:
I don't think there is any filter in my end, because every time I
downloaded about 20% of the file, then downloading paused and could
not continue any
Hi:
I have heard good things about Myrimatch and would like to compare it
with results from my usual Tandem, Mascot, and OMSSA processing in the
TPP.
The only problem is that I while I can run the program from the
command line, I am having trouble searching with anything but the
default options.
For an orbi/ltq run, myrimatch.cfg might look like:
PrecursorMzTolerance = 10
PrecursorMzToleranceUnits = ppm
FragmentMzTolerance = 0.5
FragmentMzToleranceUnits = daltons
ProteinDatabase = c:\foo\bar.fasta
NumMinTerminiCleavages = 1
CleavageRules = trypsin/p
DynamicMods = M * 15.9949 C * 57.0215
