[spctools-discuss] TPP 4.6 Tandem stops after a few files complete search.

2012-08-27 Thread Brian Hampton
I recently installed TPP 4.6 on a MacPro running Win7 64 bit. I submit 20 mzXML files for search using Tandem and after completing searching 3 - 6 of the files the pipeline stops. I have to start a new pipeline searching the unsearched files and it will halt again after searching 3-6 of the da

Re: [spctools-discuss] Using MSFileReader API to Extract Information from Thermo RAW files

2012-08-27 Thread Michael Hoopmann
Hi Josh, Not all the methods are in the old interface. You need to access the latest methods from IXRawfile5. A good example of how to use this interface is in https://proteowizard.svn.sourceforge.net/svnroot/proteowizard/trunk/pwiz/pwiz_aux/msrc/utility/vendor_api/thermo/RawFile.cpp check the

Re: [spctools-discuss] Using MSFileReader API to Extract Information from Thermo RAW files

2012-08-27 Thread Mark Wingerd
Hi Josh, / Group Back=ground Questions: - How do they report "Noise" ?? - WHAT are they reporting when they report "Noise" Thanks, MAkr On Mon, Aug 27, 2012 at 2:43 PM, Joshua G wrote: > Hi! I am tying to write a C++ program to extract Mass, Intensity and Noise > information for

[spctools-discuss] Using MSFileReader API to Extract Information from Thermo RAW files

2012-08-27 Thread Joshua G
Hi! I am tying to write a C++ program to extract Mass, Intensity and Noise information for each m/z measurement for a collection of scans in a RAW file from an Orbitrap. The libraries and documentation are provided through the following link: http://sjsupport.thermofinnigan.com/public/detail.as

Re: [spctools-discuss] PTMProphetParser on Linux

2012-08-27 Thread David Shteynberg
Hello Maik, I am not sure what you mean that it removed phospho peptides. The default function for PTMProphet updates the modifications to the most probable positions, unless you used the NOUPDATE flag. Can you send the commandline you used for PTMProphet? Thanks, -David On Thu, Aug 23, 2012 a

[spctools-discuss] SpectraST precursor tolerance

2012-08-27 Thread Matthias
Hello everybody, i am currently using SpectraST for building spectra librarys, which works fine. When i am seraching my library with a set of known spectra everything is okay. But if i turn the sM parameter above 450 for testing purpose the number of results in the pep.xml get lower, if i use

[spctools-discuss] SpectraST precusor tolerance

2012-08-27 Thread Matthias
Hello everybody, i am currently using SpectraST for building peptide librarys wich works fine. Now i am trying to validate my librarys by searching against them with known spectras and so i turned the -sM param up for testing purposes. I get the strange behaviour that if i have values of more th