Re: [spctools-discuss] Double or sequential digest using Kojak in combination with PeptideProphet

2019-09-10 Thread Michael Hoopmann
Hi Zsuzsi, Could you try renaming your enzyme, "trypsin_gluc" ? I think that is the secret enzyme name that PeptideProphet recognizes for that enzyme combination. Sorry for the trouble, I'll have dig through the code to see who is at fault (Kojak or PeptideProphet) for not properly interpreting th

[spctools-discuss] Double or sequential digest using Kojak in combination with PeptideProphet

2019-09-10 Thread nzsuzsanna
Hi, I tried to analyze my DSS and DMTMM cross-linking data using Kojak and PeptideProphet. Kojak run properly, however Peptideprophet claims for Trypsin GluC digestion, which is not defined. Kojak could handle it. Why does PeptideProphet need the enzyme info at all? Is this possible to define f