Re: [spctools-discuss] PTMProphet MSFragger modification missing mass

2020-04-07 Thread 'David Shteynberg' via spctools-discuss
Great! Although when you set MINPROB=0 the software is spending a lot of time evaluating PSMs you will never use, and some of them might have many mods causing a combinatorial expansion of peptide candidates the must be evaluated (although this is less of an issue for MASSDIFFMODE.) If you set thi

Re: [spctools-discuss] PTMProphet MSFragger modification missing mass

2020-04-07 Thread Alex Zelter
Hi David, Using MINPROB=0 solved this problem. Thanks! Alex On Monday, April 6, 2020 at 4:11:47 PM UTC-7, David Shteynberg wrote: > > Hi Alex, > > PTMProphet by default will ignore any spectrum with less than 90% > probability (iProphet is used when available.) You can change this by > setting