Re: [spctools-discuss] When to use "Decoy hits to pin down negative distribution" in PeptideProphet

2021-01-19 Thread 'David Shteynberg' via spctools-discuss
Dear Soroush, This option makes use of the TPP's semi-supervised learning mode, which can be used in conjunction with semi-parametric mode, at the user's discretion. Until now, some search engines supported by TPP analysis could only be modeled by PeptideProphet using semi-parametric and semi-sup

Re: [spctools-discuss] Error generation while converting raw files

2021-01-19 Thread 'David Shteynberg' via spctools-discuss
Dear Giangiacomo, Thanks for trying the TPP and reporting the problem. TPP uses the proteowizard's msconvert tools for this step. You can remedy the problem by either upgrading to a newer version of proteowizard's msconvert tool or use --ignoreUnknownInstrumentError option with your current ver

[spctools-discuss] Error generation while converting raw files

2021-01-19 Thread giangiacomo beretta
Hi, when I try to convert raw files, the conversion tool stops immediately reporting the following error: Reader_Thermo::fillInMetadata] unable to parse instrument model; please report this error to the ProteoWizard developers with this information: model(Orbitrap Eclipse) name(Orbitrap Eclipse