Hello Priti, You can specify non-enzymatic cleavage by setting the *protein, cleavage site* parameter to *[X]|[X]* .
More info at the X!Tandem site: https://www.thegpm.org/TANDEM/api/pcs.html https://www.thegpm.org/TANDEM/api/pcsemi.html Hope this helps, --Luis On Wed, Aug 3, 2022 at 10:59 PM Priti Panchal <panchalpriti...@gmail.com> wrote: > Hello > I am trying to do tandem analysis. I am uncertain whether we can perform a > tandem analysis for the lc-ms/ms data of non enzymatically digested sample > since in the tandem parameter file there is not any option available for > "no cleavage" or "no cut" as found in comet parameters. In tandem, there is > "semi tryptic" digestion option only. > If we can perform the analysis in tandem please suggest how to edit the > parameter file to perform analysis of non digested data. > Thank you > Priti > > -- > You received this message because you are subscribed to the Google Groups > "spctools-discuss" group. > To unsubscribe from this group and stop receiving emails from it, send an > email to spctools-discuss+unsubscr...@googlegroups.com. > To view this discussion on the web visit > https://groups.google.com/d/msgid/spctools-discuss/7cef10d1-de04-4062-a070-36eb39353adan%40googlegroups.com > <https://groups.google.com/d/msgid/spctools-discuss/7cef10d1-de04-4062-a070-36eb39353adan%40googlegroups.com?utm_medium=email&utm_source=footer> > . > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to spctools-discuss+unsubscr...@googlegroups.com. To view this discussion on the web visit https://groups.google.com/d/msgid/spctools-discuss/CACyS9brdRc6_5-X3d-6bcMTgGd%3DjQoisrFxQ4uxy0Wjv0hbUfQ%40mail.gmail.com.
