Re: [spctools-discuss] protein validation

2023-05-05 Thread felipe velasquez
nderstand > the analysis and tools well enough that you need to search a smaller > database, minimally use 500 or 1000 protein sequences and include your > protein of interest. > > Jimmy > > On Wed, May 3, 2023 at 9:41 PM felipe velasquez > wrote: > >> Hello

Re: [spctools-discuss] protein validation

2023-05-03 Thread felipe velasquez
you use decoys? > Contaminants? >- Does the virus protein contain peptides that may be shared with >proteins in the sample itself? Do you observe unique/proteotypic peptides >from that protein? > > Cheers, > --Luis > > > > On Sun, Apr 30, 2023 at 7:13 PM

[spctools-discuss] protein validation

2023-04-30 Thread felipe velasquez
Hello I have a question about protein validation. I am trying to use TPP to identify post-translational modifications of certain proteins. I performed a Comet search using the sequence of my protein of interest. However, while exploring another database, I performed a search using a protein that i

Re: [spctools-discuss] Problem with PTMprophet

2023-04-29 Thread felipe velasquez
ile to this pepXML? > Do you get any other errors or warnings before this step? Have you been > able to run other data as a test of your TPP install? > > Cheers, > --Luis > > > On Fri, Apr 28, 2023 at 11:25 AM felipe velasquez > wrote: > >> Hello luis, thanks.

Re: [spctools-discuss] Problem with PTMprophet

2023-04-28 Thread felipe velasquez
gt; We do note that in some instances other software does tend to inflate the > number of results provided to the user, albeit with either a very high or > otherwise poorly characterized error rate. > > Again, without knowing specifics, it is impossible to tell what could be > going

[spctools-discuss] Problem with PTMprophet

2023-04-27 Thread felipe velasquez
hello again I am trying to analyze some samples from thermo raw data, but I cannot find any results. However, another person analyzed the same data using another platform and found some results. Is this normal? Felipe -- You received this message because you are subscribed to the Google Groups

[spctools-discuss] Re: TPP Error

2021-04-24 Thread felipe velasquez
hi, it seems I have the same problem. were you able to find a solution? El miércoles, 27 de mayo de 2020 a las 13:36:53 UTC-4, Nimai Charan Mahanandia escribió: > I am trying to convert the Bruker .baf file to mzML format. However, it is > not converting and showing error. > > Can anybody help