Re: [spctools-discuss] 2 libra questions

2012-02-29 Thread Jimmy Eng
Rich, Since no one else has responded, I'm staring at iTRAQ data I just processed through Libra today and the intensities reported in the pepXML file are exactly those that are in the corresponding mzXML. I have never seen data where this is not the case which means something went wrong

[spctools-discuss] 2 libra questions

2012-02-27 Thread greener
Hi everyone, I have two questions about the iTRAQ quantitation by libra. (1)  Why are the iTRAQ label intensities of peptides in the Libra output files different from the intensities in the native ms/ms spectrum? (2)  Suppose a single precursor ion triggers two MS/MS scans (two HCD- CID pair).