Dear David,
Thanks for explaining the principle of multiple match. I was asking if
there is any flag in spectraST that returns only top match.
eg Protein= 1/NP_001447.2|gn|FLNA:2316|
instead of all four
4/NP_001448.2|gn|FLNB:2317|/NP_001157791.1|gn|FLNB:2317|/NP_001449.3|gn|FLNC:2318|/NP_00144
Hi Shubham,
Thanks for using the TPP tools and submitting your question here. A peptide
may map to more than one protein, when this happens the TPP will usually
map each peptide to all proteins where it can occur. There are some
exceptions to this such as I/L substitutions and protein specific co
Hi,
I am using RefSeq database which has isoforms. My pipeline includes
Mascot, peptide prophet, iProphet and spectraST.
In the spectraST output .sptxt file, for some spectra there are multiple
proteins identified.
eg.
Protein=4/NP_001448.2|gn|FLNB:2317|/NP_001157791.1|gn|FLNB:2317|/NP_00