Hello Priti,
You can specify non-enzymatic cleavage by setting the *protein, cleavage
site* parameter to *[X]|[X]* .
More info at the X!Tandem site:
https://www.thegpm.org/TANDEM/api/pcs.html
https://www.thegpm.org/TANDEM/api/pcsemi.html
Hope this helps,
--Luis
On Wed, Aug 3, 2022 at 10:59 PM
Hello
I am trying to do tandem analysis. I am uncertain whether we can perform a
tandem analysis for the lc-ms/ms data of non enzymatically digested sample
since in the tandem parameter file there is not any option available for
"no cleavage" or "no cut" as found in comet parameters. In tandem,