Hi Ray, I suspect that SpectraST will recognize this notation:
n[42]MFLVNSFLK N terminal modifications are shown after an n. Side-chain modifications are shown after the residue. Eric *From:* spctools-discuss@googlegroups.com <spctools-discuss@googlegroups.com> *On Behalf Of *RayS *Sent:* Saturday, April 25, 2020 3:01 PM *To:* spctools-discuss <spctools-discuss@googlegroups.com> *Subject:* [spctools-discuss] SpectrasT can't recognize Protein N-term acetylation modification I'm using SpectraST in TPP for building a splib format file from a bibliospec MS2 format file. While converting it issues error message for peptides with Protein N-term modifications *"MS2 IMPORT: Peptide ID has unknown modification: "M[+42.01056]FLVNSFLK". Skipped spectrum"* I guess I can write this as user defined modification in a file and specify the file name with *-M *option in the command line *M[n]|+42.01056|Protein_N-term* and the following command *spectrast.exe -cNmyLib -M SpectraST_MyMod.txt myLib.ms2* I guess the syntax I wrote is wrong because SpectraST still skips those modifications. Can anyone help me with the correct synatx and command please? Thanks -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to spctools-discuss+unsubscr...@googlegroups.com. To view this discussion on the web visit https://groups.google.com/d/msgid/spctools-discuss/c7153b98-145d-435f-bcb4-75b5dca2a893%40googlegroups.com <https://groups.google.com/d/msgid/spctools-discuss/c7153b98-145d-435f-bcb4-75b5dca2a893%40googlegroups.com?utm_medium=email&utm_source=footer> . -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to spctools-discuss+unsubscr...@googlegroups.com. To view this discussion on the web visit https://groups.google.com/d/msgid/spctools-discuss/b6e7d1fc524042e017525993e70b72e0%40mail.gmail.com.
