>Darkfield microscopy is accomplished by using a special condenser. The >condenser delivers a hollow cone of light with the tip of the cone focused >on the specimen. The angle of the light rays is such that all of the light >misses the microscope objective, thus causing a dark field of view. >However, a specimen can refract or reflect some of the impinging light and >divert it into the microscope objective. Therefore, objects in the specimen >appear to be bright against a dark background. >With this type of specimen illumination it is possible to detect objects >even when they are smaller than the resolution limit of the microscope. >However, this does not mean that you can measure their size, at least not >directly.
One thing that is possable is checking the sizes of particals with the darkfield. If we want a uniform size partical and if it is viewed to be many different sizes under the darkfield then we know that something has to be changed. If we know that we need .001 and we know that it's not possable to see .001 under the m-scope but we see particals then we know that they aren't .001. So we take a picture filter and look again. Now here's something else to consider. What would happen if a person used inferred light? What would happen to the ability to view partical size then? Take Care Reid -- The silver-list is a moderated forum for discussion of colloidal silver. To join or quit silver-list or silver-digest send an e-mail message to: silver-list-requ...@eskimo.com -or- silver-digest-requ...@eskimo.com with the word subscribe or unsubscribe in the subject: line. To post, address your message to: silver-list@eskimo.com List maintainer: Mike Devour <mdev...@id.net>
