Recently, I saw an article about a Minnesota doctor who treated an extremely difficult cast of C-difficile with a "stool transplant."
Basically, the husband's stool sample was liquified and strained and inserted into the woman's stomach through a tube. I suppose one could also drink it... This worked miraculoulsy and almost instantly. See article, below, one of many articles: Dan http://scienceblogs.com/aetiology/2007/12/fecal_transplants_to_cure_clos.php Fecal Transplants to Cure Clostridium Difficile Infection Category: Antibiotic resistance • Ecology • General Epidemiology • Infectious disease • Public health • Various bacteria Posted on: December 17, 2007 1:50 PM, by Tara C. Smith In my field, many things that cause the average man-on-the-street to get a bit squeamish or squicked are rather commonplace. My own studies include two types of bacteria that are carried rectally in humans (and other animals), so I spend an absurd amount of time thinking about, well, shit, and the lifeforms that inhabit it and collectively make up our normal gut flora. The vast majority of these species don't harm us at all, and many are even beneficial: priming our immune system; assisting in digestion; and filling niches that could be colonized by their nastier bacterial brethren. It's typically when there's some disturbance in these flora that bad things happen. For example, you may ingest food contaminated with a foreign bacterial strain that may transiently colonize your intestines, resulting in cramping and diarrhea. Typically these infections are self-limited and your normal flora "resets itself" after a short time, but some pathogenic bacteria have a propensity for making themselves at home in your gut. How to get rid of these nasty invaders then? Antibiotics are one option, but they also kill your regular bacteria, potentially making the problem worse (especially if the nasty invader happens to be resistant to many antibiotics). There has been a large increase in the use of probiotics--formulations designed to add beneficial bacteria to your gut. However, these have largely not been rigorously tested or regulated, so it's unsure how well they actually work. What if, instead of re-constitituing healthy gut flora one species at a time, you could simply take the entire fecal contents from a healthy person and use it to re-colonize your own gut--in other words, undergo a fecal transplant? Yes, it's like probiotics on steroids: getting an infusion of someone else's gut flora in order to re-establish a healthy gut ecology of your own, and squeeze out some potentially harmful organisms along the way. A recent story discusses this treatment for patients suffering Clostridium difficile infections in Scotland, but it's actually not brand-new, and has already surfaced in the peer-reviewed literature. More after the jump... First, a quick review of C. difficile, which is a spore-forming bacterium carried asymptomatically by a small percentage of us. This species has become a problem in recent years due to both the emergence of a new, more virulent strain, and apparently due to an increase in use of a certain class of antibiotics, the fluoroquinolones. These antibiotics are termed "broad spectrum:" they kill a number of different species of bacteria in one fell swoop. This is good for the clinician, because it means they can start treatment quickly, before culture results are even back from the lab. However, it may be bad for the patient in the long term because it means that the antibiotic regimen will kill not only the bacterium causing the disease, but also will wipe out many beneficial organisms in and on the body. The result can be a disturbance in the ecology of one's normal flora, setting the stage for an invader such as C. difficile to come in and set up shop--and once it's there, it's notoriously difficult to get rid of. And once it's there and causing a symptomatic infection, it can be hell to deal with, resulting in copious and sometimes frequent diarrhea, and occasionally causes a more serious and painful condition called colitis (inflammation of the colon). Additional antibiotics can eliminate C. difficile, but they don't work for all patients, and infection can result in miserable symptoms. Thus, some have turned to the fecal transplant as a last-ditch effort to cure themselves of the infection. This procedure was described in a 2003 Clinical Infectious Diseases paper, documenting 19 patients who'd undergone a fecal transplant between 1994 and 2002. Donor feces are provided to the patient via a nasogastric tube as depicted in the picture to the right. First, of course, donor stool must be procured. When possible, they used donor stool from someone the recipient would be in contact with anyway--a spouse or other household member, preferably. A fresh sample is obtained and then, um, processed. The authors describe their methods (emphasis mine): Select a stool specimen (preferably a soft specimen) with a weight of 30 g or a volume of 2 cm^3. Add 50-70 mL of sterile 0.9 N NaCl to the stool sample and homogenize with a household blender. Initially use the low setting until the sample breaks up; then, advance the speed gradually to the highest setting. Continue for 2-4 min until the sample is smooth. Filter the suspension using a paper coffee filter. Allow adequate time for slow filtration to come to an end. Refilter the suspension, again using a paper coffee filter. As before, allow adequate time for slow filtration. 25 mL of the suspension is then transferred to the recipient, who's already been prepared for the transplant via treatment with vancomycin (to kill off as much existing C. difficile as possible) and omeprazole (Prilosec, to decrease stomach acid production). The tube is then flushed with a salt solution and removed, and the recipient is free to go. (They were followed up either via phone or return visits to the clinic). Was it worth it? It appeared to be a fairly successful procedure: After the stool transplantation, 14 of the 16 surviving patients submitted a total of 20 stool samples that were tested for C. difficile toxin. Patients 3 and 11 did not submit stool samples after undergoing stool transplantation. A telephone follow‐up conversation with patient 3 and a review of the clinical record for patient 11 verified that neither patient had experienced a recurrence of diarrhea after the stool transplantation. Both patients remained free of diarrhea during the 90‐day follow‐up period. One additional patient did develop diarrhea due to C. difficile, and was given an additional course of vancomycin (after which he was fine). Additionally, "all surviving patients reported that bowel habits returned to the functional pattern that had preceded their first episode of C. difficile colitis." The authors acknowledge that additional research needs to be done (including clinical trials). A limitation exists in the design: one can't be certain if it was the vancomycin treatment or the fecal transplant that led to the resolution of symptoms (although the former seems unlikely, since recipients were all patients who had previously received vancomycin treatment). Additionally, there's obviously a lot of "ick" factor that needs to be overcome. There are also several logistic hurdles, such as storing and transporting feces in some cases: "I had to collect stool samples for five days prior to our leaving Toronto, and I collected it in an ice cream container and kept it in the fridge," said [donor] Sinukoff. She had to then fly the samples to Calgary so that [Calgary physician Dr. Tom] Louie could transplant it into her sister -- a process that involved getting the sample through airport security. "My biggest fear was that my samples were not allowed to be frozen, so I had to take them as carry-on luggage in the airplane and I was terrified that I was going to be asked to have my luggage searched," she said. As the article quips, in the annals of medical history, this method has the potential to be one of the most effective, but also most stomach-churning. Reference Aas, J. et al. 2003. Recurrent Clostridium difficile Colitis: Case Series Involving 18 Patients Treated with Donor Stool Administered via a Nasogastric Tube. CID. 36:580-585. Link. Image from http://www.disaboom.com/getfile/6e427b25-7801-4474-b794-fe8e5aab179c/NasogastricTube.aspx On Wed, Jan 20, 2010 at 7:15 AM, <martsmai...@aol.com> wrote: > > > Anyone know how to treat someone with C. difficile Clostridium difficile, > often called C. difficile or "C. diff," is a bacterium that can cause > symptoms ranging from diarrhea to life-threatening inflammation of the > colon. Illness from C. difficile most commonly affects older adults in > hospitals or in long term care facilities and typically occurs after use of > antibiotic medications. > > -- The Silver List is a moderated forum for discussing Colloidal Silver. Instructions for unsubscribing are posted at: http://silverlist.org To post, address your message to: silver-list@eskimo.com Address Off-Topic messages to: silver-off-topic-l...@eskimo.com The Silver List and Off Topic List archives are currently down... List maintainer: Mike Devour <mdev...@eskimo.com>
