Dear Brian Pratt
   I have zip up the pepXML and fasta files to Analyze_Proteins_Error.rar
and send it to ftp://insilicos.serveftp.net/pub. Thank you very much.  I
will waiting your replay.
Best wish
Bright

2009/7/15 Brian Pratt <brian.pr...@insilicos.com>

>
> Hard to say what is happening there - you might zip up the pepXML and fasta
> files and FTP them to ftp://insilicos.serveftp.net/pub and I could take a
> quick look.
>
> Brian
>
> -----Original Message-----
> From: spctools-discuss@googlegroups.com
> [mailto:spctools-disc...@googlegroups.com] On Behalf Of Bright
> Sent: Monday, July 13, 2009 10:52 PM
> To: spctools-discuss
> Subject: [spctools-discuss] Why not run Analyze Proteins when sample label
> by iTRAQ
>
>
> Hi everyone
>   I use TPP v4.2 JETSTREAM rev 1, Build 200904071723 to analyse the
> sample which when label by iTRAQ but the Analyze Protein can't normal
> finish. The error report is following:
>
> running: "C:/Inetpub/tpp-bin/PepXMLViewer.cgi -I c:/Inetpub/wwwroot/
> ISB/data/LiuXiaoHui/interact.pep.xml"
> command completed in 1 sec
>
> running: "C:/Inetpub/tpp-bin/ProteinProphet "interact.pep.xml"
> "interact.prot.xml" XML"
> ProteinProphet (C++) by Insilicos LLC and LabKey Software, after the
> original Perl by A. Keller (TPP v4.2 JETSTREAM rev 1, Build
> 200904071723 (MinGW))
>  (xml input) (report Protein Length) (using degen pep info)
> . . . reading in c:/Inetpub/wwwroot/ISB/data/LiuXiaoHui/
> interact.pep.xml. . .
> . . . read in 1 1+, 83 2+, 20 3+, 0 4+, 0 5+, 0 6+, 0 7+ spectra with
> min prob 0.05
>
> command "C:/Inetpub/tpp-bin/ProteinProphet "interact.pep.xml"
> "interact.prot.xml" XML" failed: Unknown error
>
> command "C:/Inetpub/tpp-bin/ProteinProphet "interact.pep.xml"
> "interact.prot.xml" XML" exited with non-zero exit code: -1073741676
> QUIT - the job is incomplete
>
> command "C:\Inetpub\tpp-bin\xinteract.exe -Ninteract.pep.xml -p0.05 -
> l7 -Ofp -Lcondition.xml-1 c:/Inetpub/wwwroot/ISB/data/LiuXiaoHui/
> F003217.pep.xml" failed: Unknown error
> Command FAILED
>
> Can you tell me the reasons why happend it. I will appreciate for your
> help.
>
> Thank your
>
> Bright
>
>
>
> >
>

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