I'm not familiar with that error message but possibly your search didn't run? The .out files should have scores and peptide sequences in them and not be the same format as the .dta peak lists. If they are the same, something is wrong.
On Wed, Mar 3, 2010 at 7:08 AM, BIackEye <philippschre...@web.de> wrote: > My .out Data looks just like the .dta I posted. > But I found a sequest.txt file inside my .out DIR with the following > lines: > > SEQUEST v.28, (c) 1998-2009 > > Failed to initialize FLEXnetWrapper > > On 25 Feb., 17:29, Jimmy Eng <jke...@gmail.com> wrote: >> There is no date/version information within the dta peak lists file. >> The real question is what do your Sequest search result .out files >> look like that were used as input to generate the pep.xml? >> >> On Thu, Feb 25, 2010 at 4:22 AM, BIackEye <philippschre...@web.de> wrote: >> > I'm sry, >> >> > didnt realize i was writing in german into an english forum. >> > Anyway, maybe there is sth wrong with my dta, cause there is absolutly >> > no stamps (Date, Version etc.) on the top. >> > I read in other posts, that the pepXML converter is pretty static >> > about the files he accepts for dta's. >> > Maybe there is sth buggy with my Sequest search engine. >> >> > with best regards >> > Blacky >> >> > On 22 Feb., 18:13, Jimmy Eng <jke...@gmail.com> wrote: >> >> Die pepXML Akte ist zu klein. Es gibt keine Suchresultate in der Akte. >> >> Fokus auf, wie diese Akte erzeugt wurde. >> >> >> (I thought it would be cute to reply in German. The above is a babel >> >> fish translation of: "The pepXML file is too small. There are no >> >> search results in the file. Focus on how that file was generated.") >> >> >> On Mon, Feb 22, 2010 at 8:05 AM, BIackEye <philippschre...@web.de> wrote: >> >> > Ich habe ein Problem mit den pepXML Dateien. Irgendwie sehen die zu >> >> > klein aus, und die Analyse startet auch nicht. >> >> > Hier mal der Fehlerbericht: >> >> > --------------------------------------------------------------------------------------------------------- >> >> > # Commands for session ZBTP4364S on Mon Feb 22 16:57:49 2010 >> >> > # BEGIN COMMAND BLOCK >> >> > ###### BEGIN Command Execution ###### >> >> > [Mon Feb 22 16:57:49 2010] EXECUTING: run_in c:/Inetpub/wwwroot/ISB/ >> >> > data; c:\Inetpub\tpp-bin\xinteract -Ninteract.pep.xml -p0.05 -l7 -O >> >> > c:/Inetpub/wwwroot/ISB/data/0010-100208NiLeGb01TD.pep.xml >> >> > OUTPUT: >> >> >> > c:\Inetpub\tpp-bin\xinteract (TPP v4.3 JETSTREAM rev 0, Build >> >> > 200908071234 (MinGW)) >> >> >> > running: "C:/Inetpub/tpp-bin/InteractParser "interact.pep.xml" "c:/ >> >> > Inetpub/wwwroot/ISB/data/0010-100208NiLeGb01TD.pep.xml" -L"7"" >> >> > file 1: c:/Inetpub/wwwroot/ISB/data/0010-100208NiLeGb01TD.pep.xml >> >> > processed altogether 0 results >> >> >> > results written to file c:/Inetpub/wwwroot/ISB/data/interact.pep.xml >> >> >> > direct your browser tohttp://localhost/ISB/data/interact.pep.shtml >> >> >> > command completed in 1 sec >> >> >> > running: "C:/Inetpub/tpp-bin/PeptideProphetParser "interact.pep.xml" >> >> > MINPROB=0.05" >> >> > (SEQUEST) (icat) >> >> > MS Instrument info: Manufacturer: Thermo Scientific, Model: LTQ >> >> > Orbitrap Discovery, Ionization: NSI, Analyzer: FTMS, Detector: unknown >> >> >> > command "C:/Inetpub/tpp-bin/PeptideProphetParser "interact.pep.xml" >> >> > MINPROB=0.05" failed: Unknown error >> >> >> > command "C:/Inetpub/tpp-bin/PeptideProphetParser "interact.pep.xml" >> >> > MINPROB=0.05" exited with non-zero exit code: 255 >> >> > QUIT - the job is incomplete >> >> >> > command "c:\Inetpub\tpp-bin\xinteract -Ninteract.pep.xml -p0.05 -l7 -O >> >> > c:/Inetpub/wwwroot/ISB/data/0010-100208NiLeGb01TD.pep.xml" failed: >> >> > Unknown error >> >> > END OUTPUT >> >> > RETURN CODE:65280 >> >> > ###### End Command Execution ###### >> >> > # All finished at Mon Feb 22 16:57:52 2010 >> >> > # END COMMAND BLOCK >> >> > ----------------------------------------------------------------------- >> >> > Hier meine pep,pep.xml und eine tga Datei: >> >> > [url=http://uploading.com/files/ >> >> > b12b2ac4/0010-100208NiLeGb01TD.pep.xml/] Download >> >> > 0010-100208NiLeGb01TD.pep.xml for free on uploading.com[/url] >> >> > [url=http://uploading.com/files/ >> >> > fm325f39/0010-100208NiLeGb01TD.pep.xml/] Download >> >> > 0010-100208NiLeGb01TD.pep.xml for free on uploading.com[/url] >> >> > [url=http://uploading.com/files/61md3e1d/0010-100208NiLeGb01TD. >> >> > 00331.00331.3.dta/] Download 0010-100208NiLeGb01TD.00331.00331.3.dta >> >> > for free on uploading.com[/url] >> >> >> > -- >> >> > You received this message because you are subscribed to the Google >> >> > Groups "spctools-discuss" group. >> >> > To post to this group, send email to spctools-disc...@googlegroups.com. >> >> > To unsubscribe from this group, send email to >> >> > spctools-discuss+unsubscr...@googlegroups.com. >> >> > For more options, visit this group >> >> > athttp://groups.google.com/group/spctools-discuss?hl=en. >> >> > -- >> > You received this message because you are subscribed to the Google Groups >> > "spctools-discuss" group. >> > To post to this group, send email to spctools-disc...@googlegroups.com. >> > To unsubscribe from this group, send email to >> > spctools-discuss+unsubscr...@googlegroups.com. >> > For more options, visit this group >> > athttp://groups.google.com/group/spctools-discuss?hl=en. > > -- > You received this message because you are subscribed to the Google Groups > "spctools-discuss" group. > To post to this group, send email to spctools-disc...@googlegroups.com. > To unsubscribe from this group, send email to > spctools-discuss+unsubscr...@googlegroups.com. > For more options, visit this group at > http://groups.google.com/group/spctools-discuss?hl=en. > > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. 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