as far as I know, the TPP quantification tools currently do not support triplex SILAC analysis.
On Wed, Apr 4, 2012 at 2:58 AM, zeyu sun <szy3...@gmail.com> wrote: > Dear TPP team, > I wondering if I can get help from you to process one of my SILAC data set. > My experiment design is a SILAC-like scheme. But unlike the traditional > SILAC design which contain L-Arg, L-Lys (no isotopes) as the light label, > and H-Arg(Arg10, with 13C6 15N4) and H-Lys( Lys8 with 13C6 15N2) as the > heavy label, my triplex design contain L-Arg, L-Lys (no isotopes) as the > light label, M-Arg(Arg4, with 15N4) and M-Lys( Lys2 with 15N2) as the > medium label, H-Arg(Arg10, with 13C6 15N4) and H-Lys( Lys6 with 13C6) as the > heavy label. I noticed in MS scan spectra, most medium labeled peaks are > mixed with isotopic peaks from the light labeled precursors, the M and L > peaks are just 2 or 4 Da away. Is there any way I can deconvolute those > peaks before database (X!tandem or Mascot) search when I convert .RAW to > mzXML? does the subsequent TPP workflow support those data format? > thank you very much! > > SunSun > > -- > You received this message because you are subscribed to the Google Groups > "spctools-discuss" group. > To post to this group, send email to spctools-discuss@googlegroups.com. > To unsubscribe from this group, send email to > spctools-discuss+unsubscr...@googlegroups.com. > For more options, visit this group at > http://groups.google.com/group/spctools-discuss?hl=en. -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to spctools-discuss@googlegroups.com. To unsubscribe from this group, send email to spctools-discuss+unsubscr...@googlegroups.com. For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en.
