Hello all, Thank you in advance for your help. I have been using MS-GF+ to search spectra prior to analysis with TPP. I had to create a new set of scoring parameters for MS-GF+ in order to handle Orbitrap Fusion combined CID/ETD data, however after doing so, Peptide Prophet always errors out with 0 hits following the accurate mass model and PepXMLViewer crashes. I am aware that this can be indicative of bad data, yet my previous scoring parameters had no problem dealing with this same dataset. I'm under the impression that this is an issue with the settings used to generate those new scoring parameters with MS-GF+, but I unfortunately I have not found a better location to seek assistance with this.
If anyone could point me in the right direction for resolving this, I would greatly appreciate it. Ryan Lumpkin Graduate Student University of California, San Diego -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to spctools-discuss+unsubscr...@googlegroups.com. To post to this group, send email to spctools-discuss@googlegroups.com. Visit this group at http://groups.google.com/group/spctools-discuss. For more options, visit https://groups.google.com/d/optout.
